TRAIL contributes to the apoptotic effect of 13-cis retinoic acid in human sebaceous gland cells

Riferimento: 
Br J Dermatol. 2011 Sep;165(3):526-33.
Autori: 
Nelson AM, Cong Z, Gilliland KL, Thiboutot DM.
Fonte: 
Br J Dermatol. 2011 Sep;165(3):526-33.
Anno: 
2011
Azione: 
Il meccanismo di azione dell' acido 13-cis retinoico nel trattamento dell' acne è sconosciuto. 13-cRA attiva i geni chiave coinvolti nell' apoptosi, presenti nei sebociti, tra cui il fattore di necrosi tumorale (TRAIL).
Target: 
13-cRA/ghiandole sebacee.

ABSTRACT
BACKGROUND
:
The full mechanism of action of isotretinoin [13-cis retinoic acid (13-cis RA)] in treating acne is unknown. 13-cis RA induces key genes in sebocytes that are involved in apoptosis, including Tumor necrosis factor Related Apoptosis Inducing Ligand (TRAIL). OBJECTIVES:
In this study, we investigated the role of 13-cis RA-induced TRAIL within SEB-1 sebocytes.
METHODS:
Using 13-cis RA and recombinant human TRAIL (rhTRAIL) protein, we assessed induction of TRAIL and apoptosis in SEB-1 sebocytes, normal keratinocytes and patient skin biopsies.
RESULTS: Treatment with rhTRAIL protein increased TUNEL-positive staining in SEB-1 sebocytes. TRAIL siRNA significantly decreased the percentage of TUNEL-positive SEB-1 sebocytes in response to 13-cis RA treatment. Furthermore, TRAIL expression increased in the skin of patients with acne after 1 week of isotretinoin therapy compared with baseline. TRAIL expression localized within sebaceous glands. Unlike sebocytes, TRAIL protein expression was not increased in normal human epidermal keratinocytes in response to 13-cis RA, nor did rhTRAIL induce apoptosis in keratinocytes, suggesting that TRAIL is key in the sebocyte-specific apoptotic effects of 13-cis RA.
CONCLUSIONS: Taken together, our data suggest that TRAIL, like the neutrophil gelatinase-associated lipocalin, is involved in mediating 13-cis RA apoptosis of sebocytes. Free PMC Article

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