Melatonin enhances DNA repair capacity possibly by affecting genes involved in DNA damage responsive pathw

Riferimento: 
BMC Cell Biol. 2013 Jan 7;14:1.
Autori: 
Liu R, Fu A, Hoffman AE, Zheng T, Zhu Y.
Fonte: 
BMC Cell Biol. 2013 Jan 7;14:1.
Anno: 
2013
Azione: 
Melatonin enhance DNA repair capacity by affecting several key genes involved in DNA damage responsive pathways.
Target: 
DNA repair capacity

Free PMC Article
Abstract
BACKGROUND:
Melatonin, a hormone-like substance involved in the regulation of the circadian rhythm, has been demonstrated to protect cells against oxidative DNA damage and to inhibit tumorigenesis.
RESULTS:
In the current study, we investigated the effect of melatonin on DNA strand breaks using the alkaline DNA comet assay in breast cancer (MCF-7) and colon cancer (HCT-15) cell lines. Our results demonstrated that cells pretreated with melatonin had significantly shorter Olive tail moments compared to non-melatonin treated cells upon mutagen (methyl methanesulfonate, MMS) exposure, indicating an increased DNA repair capacity after melatonin treatment. We further examined the genome-wide gene expression in melatonin pretreated MCF-7 cells upon carcinogen exposure and detected altered expression of many genes involved in multiple DNA damage responsive pathways. Genes exhibiting altered expression were further analyzed for functional interrelatedness using network- and pathway-based bioinformatics analysis. The top functional network was defined as having relevance for "DNA Replication, Recombination, and Repair, Gene Expression, [and] Cancer".
CONCLUSIONS:
These findings suggest that melatonin may enhance DNA repair capacity by affecting several key genes involved in DNA damage responsive pathways.

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