ABSTRACT
Icb-1 (C1orf38) is a human gene initially described by our group to be involved in differentiation processes of cancer cells. To further elucidate the function of the icb-1 gene in differentiation of breast and endometrial cancer cells, we knocked down its expression by means of shRNA transfection. Knockdown of icb-1 inhibited the vitamin D3-induced up-regulation of E-cadherin expression in both MCF-7 and HEC-1B cells. Induction of E-cadherin expression by all-trans retinoic acid (ATRA) was also blocked in both cell lines expressing icb-1 siRNA. Examination of icb-1 and E-cadherin expression in 66 breast cancer tissue samples revealed a significant positive correlation between the two genes. In MCF-7 cells, silencing of the icb-1 gene inhibited the ATRA- and the vitamin D3-induced up-regulation of lactoferrin and estrogen receptor β expression. The data of our knockdown study suggest that icb-1 may act as a mediator of differentiation signals in breast cancer cells induced by ATRA or vitamin D3. These findings together with the observed co-expression of icb-1 with E-cadherin in breast cancer samples support an important role of the icb-1 gene in cancer cell differentiation.
Silencing of the icb-1 gene inhibits the induction of differentiation-associated genes by vitamin D3 and all-trans retinoic acid in gynecological cancer cells
Riferimento:
Int J Mol Med. 2011 Jul;28(1):121-7.
Autori:
Haselberger M, Springwald A, Konwisorz A, Lattrich C, Goerse R, Ortmann O, Treeck O.
Fonte:
Int J Mol Med. 2011 Jul;28(1):121-7.
Anno:
2011
Azione:
Il gene umano icb-1 può agire come mediatore dei segnali di differenziazione indotti da acido all-trans retinoico (ATRA) o vitamina D3 nelle cellule di carcinoma mammario.
Target:
ATRA-Vit D3/carcinoma mammario.
Sostanze: